Abstract: A method for screening Etomidate and Etomidate acid in human hair by high performance liquid chromotography tandem mass spectroscopy(HPLC-MS/MS)has been established and applied for the detection of real hair samples.The hair was washed,cut,frozen and ground,added to the internal standard,extracted ultrasonic with methanol as solvent,concentrated by nitrogen blowing.The concentrated extract was reconstituted to 100 μ L by methanol for HPLC-MS/MS analysis.The correlation coefficients of etomidate and etomidate acid in the linear range(0.1～lng/mg)were 0.999 and 0.997,the recoveries were 89％～113％,and the detection limits were 0.01ng/mg and 0.02ng/mg,respectively.The method was rapid,sensitive and accurate,and could be used for qualitative and quantitative detection of the two drugs in hair at the same time.