Abstract: Body fluid stains are common evidences at crime scenes,and the identification of their sources is often crucial for case characterization. Raman spectroscopy offers advantages such as rapid analysis,non-destructive testing,and high sensitivity. When combined with chemometric methods,it enables accurate identification of body fluid stains. This paper reviews the research progress of Raman spectroscopy technology in the examination of various body fluids,including blood,semen,vaginal secretions,urine,saliva,and sweat. Through spectral characteristics and classification models,different body fluids can be identified and classified. The impacts of complex substrates and mixed stains are significant concerns in current research,which increasingly aligns the application of Raman spectroscopy with practical needs. Furthermore,improvements in analytical models allow Raman spectroscopy analysis to provide information on the species of body fluids,time since deposition,donor gender and race. This facilitates rapid assessment of the relevance of body fluid stains to cases and offers investigative leads.